Journal article
Neuroanatomical distribution of fluorophores within adult RXFP3 Cre-tdTomato/YFP mouse brain
IM Eraslan, M Egberts-Brugman, JL Read, LM Voglsanger, RM Samarasinghe, L Hamilton, P Dhar, RJ Williams, LC Walker, S Ch'ng, AJ Lawrence, AJ Walker, OM Dean, AL Gundlach, CM Smith
Biochemical Pharmacology | PERGAMON-ELSEVIER SCIENCE LTD | Published : 2024
Abstract
Relaxin-family peptide 3 receptor (RXFP3) is activated by relaxin-3 in the brain to influence arousal and related functions, such as feeding and stress responses. Two transgenic mouse lines have recently been developed that co-express different fluorophores within RXFP3-expressing neurons: either yellow fluorescent protein (YFP; RXFP3-Cre/YFP mice) or tdTomato (RXFP3-Cre/tdTomato mice). To date, the characteristics of neurons that express RXFP3-associated fluorophores in these mice have only been investigated in the bed nucleus of the stria terminalis and the hypothalamic arcuate nucleus. To better determine the utility of these fluorophore-expressing mice for further research, we characteri..
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Awarded by Monash University
Funding Acknowledgements
This study was supported by a Research Training Program Scholarship/Deakin University Postgraduate Research Scholarship awarded to Izel M. Eraslan, and internal Deakin University funding awarded to Craig M. Smith through the School of Medicine, Faculty of Health, Centre for Molecular and Medical Research Strategic Research Centre, and the Institute for Mental and Physical Health and Clinical Translation. We would also like to acknowledge the MMRRC/Gensat programme for the supply of the Tg [Rxfp3-cre mouse (RS38Gsat strain stock number 036667-UCD) , and Dr Tobias Merson (Monash University, Australia) for provision of mice from the Rosa26 YFP strain. The initial establishment and maintenance of the RXFP3-Cre/YFP and RXFP3-Cre/tdTomato mouse lines was supported by funding from the National Health and Medical Research Council of Australia, grant numbers 1024885 and 1165801 (ALG) , and 1079893 (AJL, ALG) . We acknowledge the Victorian State Government Operational Infrastructure Scheme. We also thank Dr Bowen Dempsey (Macquarie University) for providing insights included in the Discussion, and to Wiley Press and the Journal of Comparative Neurology for allowing the reproduction of RXFP3 mRNA data (Smith et al. , 2010) within Table 1 and Fig. 1 (Copyright Clearance Centre, Marketplace Order Licence ID 1404945-1) .